2 - Working with Multi-Channel Images

2 - Working with Multi-Channel Images#

2.1 - Composite images - channels display, splitting and merging#

  1. File > Open Samples > Fluorescent Cells (IJ:26.4)

  2. Image > Color > Channels Tool (IJ:28.7.5)

  3. toggle the Channel checkboxes to show/hide the respective channel

  4. change the dysplay mode:

    • using the dropdown menu, switch between Composite, Color and Grayscale modes and observe the changes in the image display

  5. select back the Composite dysplay mode

  6. Image > Color > Arrange Channels

    • click on New 1, and select Magenta to change the LUT of the first channel

  7. re-arrange the order of the channels:

    • in the New channel order settings, modify the order of the channels by changing the order of the 123 numbers (e.g. 213)

    • click OK to apply the changes

  8. split channels

    • Image > Color > Split Channels (IJ:28.5.1)

  9. merge channels to composite

    • Image > Color > Merge Channels (IJ:28.5.2)

    • check create composite checkbox (IJ:28.5.2)

  10. LUT (look-up table) (IJ:19.17 & IJ: 28.15)

    • change LUT of the channels (LUT in the startup tools or Image > Lookup Tables)

  11. color blindness

    • convert to RGB Color: Image > Type > RGB Color (IJ:7)

    • Image > Color > Simulate Color Blindness

    • Image > Color > Dichromacy

2.2 - RGB images - replace red with magenta#

  1. File > Open Samples > Fluorescent Cells (IJ:26.4)

  2. convert to RGB Color: Image > Type > RGB Color (IJ:7)

  3. Image > Color > Replace Red with Magenta