2 - Working with Multi-Channel Images

2 - Working with Multi-Channel Images#

2.1 - composite images - splitting and merging#

  1. File > Open Samples > Fluorescent Cells (IJ:26.4)

  2. Image > Color > Arrange Channels

    • click on New 1, and select Magenta

  3. split channels

    • Image > Color > Split Channels (IJ:28.5.1)

  4. merge channels to composite

    • Image > Color > Merge Channels (IJ:28.5.2)

    • check create composite checkbox (IJ:28.5.2)

  5. LUT (look-up table) (IJ:19.17 & IJ: 28.15)

    • change LUT of the channels (LUT in the startup tools or Image > Lookup Tables)

  6. color blindness

    • convert to RGB Color: Image > Type > RGB Color (IJ:7)

    • Image > Color > Simulate Color Blindness

    • Image > Color > Dichromacy